Faster process for detection of S‐nitrosylation PTM’s, efficiently blocks free thiols at pH 4.0. The process avoids denaturing agents, high temperatures and pH≥ 7.0.

About

Background Protein post‐translational modification (PTM) increases the functional diversity of the proteome by the covalent addition of functional groups or proteins, proteolytic cleavage of regulatory subunits or degradation of entire proteins. PTMs influence almost all aspects of normal cell biology and pathogenesis. Therefore, identifying and understanding PTMs is critical in the study of cell biology, disease treatment and prevention. S‐Nitrosylation; the result of covalent bonding between a nitric oxide (NO) group into thiol groups to form S‐nitrosothiol (SNO) ‐ is a form of protein post‐translational modification. S‐Nitrosylation is a critical PTM used by cells to stabilize proteins and regulate gene expression. In many cases, SNO is believed to regulate protein function and activity with both physiological and pathological consequences. To date, over 3000 peptides and proteins have been characterized and studied as SNO targets. Developed in 2001(Jaffrey, et.al), the Biotin Switch Technique has become the most widely used protocol to study S‐nitrosylation. However, this tool suffers from several drawbacks including false positives if all free thiols are not blocked before SNO reduction, disulfide scrambling, and thermal degradation. Approaches to address these limitations have produced second order problems. Regardless BST and related techniques have been described in the literature as "technically challenging and labor intensive."

Key Benefits

improves on the commonly-used Biotin Switch Technique

Applications

medical research

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