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Unlike indigo carmine, this is a "turn-on" sensor and specific for ozone. Indigo carmine cannot distinguish between ozone and some other reactive oxygen species.
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Summary The inventors have developed a specific fluorescent sensor for ozone. This sensor can detect ozone in the air, in serum, and in pH 7 buffer at 24 to 37 °C by indicating bright fluorescent green. Fluorescent compounds exhibit a fluorescence signal only when the phenolic hydroxyl group is deprotenated. By starting with commercially available 2,7-dichlorofluorescein a two step synthesis of a non-fluorescent compound was prepared by addition of a 1-butenyl group to the phenolic hydroxyl group. The subsequent addition of ozone to this compound resulted in a two step reaction to give a deprotenated fluorescent compound by the elimination of acrolein. This reaction is specific to ozone and does not occur with other reactive oxygenated compounds.