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This process utilizes large panels of primers to amplify short RNA-DNA fragments into separate and off-set pools to help reconstruct the gennomic sequences.
About
Ultra-High Sensitivity RT-PCR for Viral Genome Sequencing Tech ID: UA16-197 Invention: University of Arizona researchers have developed a novel process for genome sequencing RDA-DNA fragments, such as would be present in archived or damaged biological samples. The process utilizes large panels of primers to amplify short RNA-DNA fragments into separate and off-set pools. These off-set pools of amplicons are used to reconstruct whole and near-whole genomic sequences in samples otherwise deemed non-useful. Background: When performing conventional polymerase chain reaction (PCR) testing for genomic sequencing, some poor-quality biological samples are deemed "negative" or unusable. However, this result may be caused by RNA/DNA fragments which are simply too short and fragmented, or too unique, for conventional methods to identify. This material may currently be relegated to non-analysis. This invention enables analysis of samples comprising low concentrations or degraded nucleic acid, samples with sought-after rare mutations, formalin-fixed paraffin-embedded (FFPE), or generally, any poor-quality samples where conventional PCR methods have failed.
Key Benefits
- More sensitive than conventional RT-PCR testing - Cheaper than next generation sequencing (NGS), which is expensive and limited in application - Successful in low viral load samples
Applications
- Amplification and genomic sequencing of material in biologic samples that are damaged or of poor quality - Viral or infectious disease screening - Full genomic sequencing in archive samples - Clinical diagnostics - Vaccine research - COVID-19 viral screening, diagnostics, and vaccine research