This invention relates to a new family of fluorescent ROS sensors can detect superoxide and the hydroxyl radical in living cells, tissue samples and for the first time in vivo.

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Background: This invention relates to a new family of fluorescent ROS sensors can detect superoxide and the hydroxyl radical in living cells, tissue samples and for the first time in vivo. These sensors have excellent stability to auto-oxidation, tunable emission wavelengths and nanomolar sensitivity to ROS. They will have numerous applications as research tools and medical diagnostics.

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