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This technology used to be restrictive and expensive but by combining microfluidics with in situ protein expression from a cDNA microarray addresses these limitations.
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Summary The invention relates to detection and easy quantitation of protein PTM employing a modular integrated microfluidic platform (IMPA) for multiple PTM analyses on freshly synthesized protein arrays. IMPA can be utilized for identifying targets of a specific enzyme in physiologically relevant contexts, as well as for elucidating PTM fingerprints of particular cells or tissues. Moreover, this platform may be optimized for any type of PTM that can be fluorescently tagged or immune labeled. The minute amounts of biological material and reagents required for analysis using IMPA override the inherent limitation of PTM research imposed by MS, enabling application to low-mass tissues with relevance to basic and translational science. The system is thus applicable for identification, classification, characterization and quantification of PTMs in quasicellular environments and for diagnosis of biological conditions associated with PTMs.