Optical imaging of protein

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Stanford Reference: 05-341 Abstract Stanford researchers have developed an improved sensor system that detects phosphorylation and dephosphorylation in a quantifiable manner both in vitro and in vivo. Unlike current methods that utilize phospho-specific antibodies that detect modulation events only in cell lysates, this new system has the unique capability of studying phosphorylation and dephosphorylation in cell culture and in cells of living animals using optical imaging. This technology can be applied to drug development studies in which drugs modulate phosphorylation/dephosphorylation of various proteins in living animals. Further, the use of this system in living small animal models will enhance the direct translation of drugs for clinical evaluation by fulfilling the pre-clinical validation.  Applications In vitro and in vivo examination of phosphorylation and dephosphorylation events Molecular screening of phosphorylation and dephosphorylation induction or involvement Advantages Allows for the study of phosphorylation events in cell lysates, intact cells, and living animals Accelerate drug development programs by minimizing expense, eliminating use of cumbersome evaluation strategies, and reducing time to market