Nucleic Acid Detection

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RAPID ALTERNATIVE TO DNA AGAROSE GEL ELECTROPHORESIS PCRD nucleic acid detection LFDAbingdon Health manufactures nucleic acid lateral flow immunoassays (NALFIA), called PCRD, which can be used as an alternative to using DNA agarose gel electrophoresis. While taking only 10 minutes to detect the successful amplification of target DNA sequences PCRD is the choice for busy laboratories wanting fast results.   WHAT IS PCRD NUCLEIC ACID DETECTION? PCRD Nucleic acid lateral flow assays are qualitative rapid assays designed to detect amplicons labelled with DIG/Biotin and/or FITC (or FAM)/Biotin, with the addition of a control line. DETECTION OF AMPLIFIED DNA PRODUCTS USING LATERAL FLOW ASSAYS PCRD is a rapid, safe and sensitive way to confirm the successful outcome of a DNA amplification exercise. The lateral flow test strips incorporate reagents which sequentially capture and allow the visualisation of double-stranded amplification products containing selected binding partners. This simple method of amplicon detection takes only 10 minutes, and the result is detectable by eye due to an aggregation of carbon particles at the capture lines. Advantages:  Also, PCRD is a rapid, safe and sensitive alternative to ethidium bromide staining of agarose gels. In addition to being able to cope with large workloads, owing to their ease of use, the following benefits make PCRD an obvious choice for post DNA amplification nucleic acid confirmation. Safe to use – avoids using intercalating dye and UV light No special chemistry or equipment required Time to result of 10 minutes Easy to use High sensitivity Many strips can be run simultaneously Cost effective Due to their versatility, our nucleic acid detection assays can be used in a number of applications. Examples include: Meat speciation testing Food and drink authenticity Rapid disease detection – veterinary, human and plant amongst others