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Identification, isolation and enrichment of native oligodendrocyte precursors and their oligodendrocytes daughter cells.
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Description This technology provides a strategy for identification, isolation and enrichment of native oligodendrocyte precursors and their oligodendrocytes daughter cells for their use in engraftment and transplantation and other therapeutic applications. Applications: This technology provides a method of separating oligodendrocytes or progenitor cells from a mixed population of cells based upon cell type-selective expression of cell-specific promoters (fluorescence-activated cell sorting of subcortical white matter cells transfected with fluorescent protein driven by the Oligodendrocyte-specific early promoter). Advantages: A major impediment to both the analysis of the biology of adult neural precursors and to their use in engraftment and transplantation studies has been their relatively scarcity in adult brain tissue and their consequent low yield when harvested. Current approaches toward the use of neural precursor cells have focused upon preparing clonal lines derived from single progenitors. However, such propagated lines can become progressively less representative of their parental precursors with time and passage in vitro. This technology provides a strategy for live cells identification, isolation and enrichment of native oligodendrocyte precursors and their oligodendrocytes daughter cells.