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The system enable to establish systemic infection and silencing of endogenous maize genes homologous to gene fragments inserted into the FMV viral vector.
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Foxtail Mosaic Virus Transient Gene Silencing Vector for Maize & other Monocots Summary Plant viruses have been widely used as vectors for foreign gene expression and virus-induced gene silencing (VIGS). A limited number of viruses have been developed into viral vectors for the purpose of VIGS in monocotyledonous plants and only one has been shown to infect maize. ISU researchers have developed a new DNA-based VIGS system derived from Foxtail mosaic virus (FMV) which is able to establish systemic infection and silencing of endogenous maize genes homologous to gene fragments inserted into the FMV viral vector. Proof-of-concept VIGS applications of this vector system was accomplished by silencing two genes in the sweet corn line Golden Bantam. In addition, the research demonstrated that the FMV vector establishes systemic infection in maize inbred lines, sorghum, and green foxtail, indicating the potential wide applications of this viral vector system for functional genomic studies in maize and other monocots. Advantage Monocot-specific vector Proven to work in Maize, Sorghum, and Green Foxtail Transient expression enables high-throughput Application Agricultural Research Direct Link http://isurftech.technologypublisher.com/technology/21318 Patent Patent(s) applied for Technology ID 4344