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This invention is a novel oligonucleotide-based molecular probe for imaging native, non-engineered RNA in live cells.
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Background: This invention is a novel oligonucleotide-based molecular probe for imaging native, non-engineered RNA in live cells. These probes have been demonstrated to show single molecule sensitivity, show RNA dynamics and interactions with stress granules and proteins, and the ability to target two RNA species and viral RNA. The probes can be delivered into cells using known techniques such as via steptolysin O. Target RNA is identified by the enhanced signal-to-background ration achieved through binding of multiple probes per target RNA, or through accumulation of RNA in granules. The probes are small in size, easy to assemble, and have brightness at multiple wavelengths, making them broadly applicable for studying many types of RNA-related events within cells.