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Allozymes has built a panel of unspecific peroxygenases (wild-type and optimized) that are ready to be tested and has implemented a workflow to quickly engineer UPOs in yeast.
About
Regio- and stereo-selective mono-hydroxylation of non activated carbons has always been a challenge in biocatalysis. Cytochromes P450s are not appropriate for large scale biotransformations because of the low productivity. Unspecific peroxygenases (UPOs) - EC 1.11.2.1 - is an emerging class of enzymes that can oxyfunctionalize a wide range of substrates.
Key Benefits
Due to the promiscuous nature of the enzyme it was named ‘unspecific peroxygenase/peroxidase’. The enzyme class can oxidize a diverse array of substrates in different ways, including hydroxylations, epoxidations, dealkylation reactions, or heteroatom oxygenation and has been recombinantly expressed in S. cerevisiae and Pichia pastoris and subjected to directed evolution. This enzyme class is mechanistically simpler than P450 as it requires only hydrogen peroxide as the terminal oxidant without the need for cofactors and appropriate cofactor recycling systems.
Allozymes has built a panel of unspecific peroxygenases (wild-type and optimized) that are ready to be tested and has implemented a workflow to quickly engineer UPOs in Pichia pastoris for selective mono-hydroxylation of synthetic building blocks.
Applications
Biocatalysis for the manufacturing of chemicals
Enzyme supply from gms to tons scale