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A combination of amylolytic yeast and bacteria, and their relative enzymes, for the production of bioethanol through a consolidated bioprocess of soluble and raw starch substrates.
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Brief description The invention relates to the use of a combination of amylolytic yeast and bacteria, and their relative enzymes, for the production of bioethanol through a consolidated bioprocess of soluble and raw starch substrates. Approximately only 10% of amylolytic enzymes can hydrolyse linkages in raw or unmodified starch and these enzymes usually contain starch binding domains. Target market Industrial enzyme manufacturers, bioethanol producers. Value Proposition/ Benefits The combination of alpha-amylase and glucoamylase enzymes and their host organisms is capable of hydrolyzing starches without the specific use of specialized starch binding domains in bioethanol production process. Consolidated bioprocessing in bioethanol production could be an economically viable option compared to existing methods because it incorporates the hydrolysis of the starch and its fermentation in a single step. The invention may include the use of recombinant S cerevisiae in the process as well. Innovation status A national phase application was filed for this invention. The technology and method can be demonstrated on a laboratory scale.