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The method eliminates the capture of mis-folded, aggregated, and inactive antibody molecules that cannot be differentiated by protein A/G columns.
About
Description: Novel affinity chromatography method that utilizes the Nucleotide Binding Site (NBS) as a target for selectively purifying antibodies from complex mixtures. Antibodies can be selectively captured and retained on a NBS IBA column and can successfully elute by applying a mild NaCl gradient. The NBS IBA column consistently yields >95% antibody recovery with >98% purity, even when the antibody was purified from complex mixtures such as conditioned cell culture supernatant, hybridoma media, and mouse ascites fluid. Key Features and Benefits: Lower production costs Improved batch quality Increased column durability and lifespan No leaching of protein A/G into the purified antibody. Selective capture of active antibodies only Eliminates the capture of mis-folded, aggregated, and inactive antibody molecules that cannot be differentiated by protein A/G columns.