Antibiotic Resistance Database

About

Background The Comprehensive Antibiotic Resistance Database (CARD) is a bioinformatic resource for antimicrobial resistance (AMR) surveillance in healthcare, agriculture and environmental settings. Technology Overview The CARD is an ontology- and model-based framework for detection of antibiotic resistance genes. It contains an expert-curated collection of characterized, peer-reviewed resistance determinants and associated antibiotics, organized by the Antibiotic Resistance Ontology (ARO) and AMR gene detection models. The software utilizes these detection models for predicting AMR from genome sequences. Other associated tools include the Resistance Gene Identifier (RGI) which is a novel genome analysis tool for resistome prediction. It annotates DNA and protein sequences and detects matches to CARD reference sequences, variants of known AMR genes and emergent threats. Resistomes is a computer-generated data set including genome annotation and variants data using RGI for 79 pathogens of interest. For each of these pathogens, complete chromosome sequences, plasmid sequences, and whole genome shotgun assemblies were analysed individually by RGI to generate prevalence data. Results are further categorized using the ARO, e.g. “beta-lactam resistance protein”, “antibiotic inactivation enzyme”, etc. Further Details: The CARD and associated tools are available online at card.mcmaster.ca. #15-055