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3D Bioprinting and Lamination
3D Bioprinting and Lamination Approach to Create Complex In Vitro Models
A new layer-by-layer bioprinting and lamination approach that can recapitulate the complexities of natural tissues
About
Background Bioprinting is becoming a powerful tool in tissue engineering for both whole tissue printing and development of in vitro models that can be used in drug discovery, toxicology, and in vitro bioreactors. Nevertheless, the ability to create complex 3D culture systems with different types of cells and extracellular matrix (ECM) integrated with perfusable channels has been a challenge. Technology Overview A new layer-by-layer bioprinting and lamination approach has been developed that can recapitulate the complexities of natural tissues with proper 3D distribution of cells, ECM, and perfusion channels. This scaffold-based approach results in mechanically stable 3D structures with custom-patterned multicellular distributions and embedded perfusion channels that allow easy access to the cells throughout the structure without the need to fix or section the samples. This technique provides biologists with a unique tool to perform sophisticated in vitro assays. Stage of Development: Proof of principle has been performed. #19-028
Key Benefits
• Method can accommodate a wide variety of cell, ECM and scaffold types with the ability to easily pattern multi-material combinations in a single construct • Scaffold-based approach allows fabrication of “thin” or assembly of “thicker” complex 3D structures with high mechanical stability • Closer complexity to that of biological tissues allows more accurate responses as in vitro models of cellular interactions
Applications
• Fabricate complex 2D or 3D structures with patterned cells and channels for drug discovery and toxicology applications • Evaluate cell migration, proliferation and signaling under a variety of conditions or in response to certain stimuli, e.g. different ECM types and/or presence of chemicals • Create customized 3D bioreactor and artificial organ architectures